Biological Control Potential of Trichoderma Species and Bacterial Antagonists against Sclerotinia sclerotiorum on Canola in Western Australia

Fifteen fungal and three bacterial biological control agents (F-BCA B-BCA, respectively) were isolated from the canola production areas of Western Australia to investigate their potential for controlling sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum under in vitro field conditions. The capacity these isolates inhibit mycelial growth sclerotia formation S. was assessed dual culture tests Petri dishes. Using Sanger Sequencing ITS regions, F-BCAs identified as Trichoderma atroviride (four isolates), T. gamsii (three koningiopsis (two longibrachiatum paraviridescens pseudokoningii (one isolate) viridescens isolate). Four seven species (T. koningiopsis, gamsii, viridescens) are reported first time Australia. 16S rRNA sequencing B-BCA1 B-BCA2 Serratia proteamaculans B-BCA3 Ochrobactrum anthropi. There significant differences among (P≤0.001) effect on radial (40–60% inhibition) (65–100% inhibition). Two (F-BCA12 F-BCA15) completely blocked sclerotial pathogen Potato dextrose agar + 10 ppm/L Aureomycin (PDAA). Incubation soil inoculated with F-BCA indicated that colonized conidia each F-BCA, all presence failed germinate PDAA. B-BCAs reduced 57–59% 89–95%. Selected atroviride) (O. anthropi proteamaculans) significantly disease incidence glasshouse Field efficacy tested BCAs similar or better than commercial fungicide Prosaro®. © 2022 Friends Science Publishers